30 research outputs found

    A ubiquitous service-oriented automatic optical inspection platform for textile industry

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    Within a highly competitive market context, quality standards are vital for the textile industry, in which related procedures to assess respective manufacture still mainly rely on human-based visual inspection. Thereby, factors such as ergonomics, analytical subjectivity, tiredness and error susceptibility affect the employee's performance and comfort in particular and impact the economic healthiness of each company operating in this industry, generally. In this paper, a defect detection-oriented platform for quality control in the textile industry is proposed to tackle these issues and respective impacts, combining computer vision, deep learning, geolocation and communication technologies. The system under development can integrate and improve the production ecosystem of a textile company through a properly adapted information technology setup and associated functionalities such as automatic defect detection and classification, real-time monitoring of operators, among others.This work was financed by the project “Smart Production Process” (No. POCI-01-0247-FEDER-045366), supported under the Incentive System for Research and Technological Development - Business R&DT (Individual Projects)

    Enantiopure 4‐oxazolin‐2‐ones and 4‐methylene‐2‐oxazolidinones as chiral building blocks in a divergent asymmetric synthesis of heterocycles

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    En este trabajo se describe la reactividad de las oxazolidin-2-onas en un ambiente quiral obteniĂ©ndose resultados novedosos, los cuales se describen extensamente.Enantiopure 3‐((R)‐ and 3‐((S)‐1‐phenylethyl)‐4‐oxazoline‐2‐ones were evaluated as chiral building blocks for the divergent construction of heterocycles with stereogenic quaternary centers. The N‐(R)‐ or N‐(S)‐1‐phenylethyl group of these compounds proved to be an efficient chiral auxiliary for the asymmetric induction of the 4‐ and 5‐positions of the 4‐oxazolin‐2‐one ring through thermal and MW‐promoted nucleophilic conjugated addition to Michael acceptors and alkyl halides. The resulting adducts were transformed via a cascade process into fused six‐membered carbo‐ and heterocycles. The structure of the reaction products depended on the electrophiles and reaction conditions used. Alternative isomeric 4‐methylene‐2‐oxazolidinones served as chiral precursors for a versatile and divergent approach to highly substituted cyclic carbamates. DFT quantum calculations showed that the formation of bicyclic pyranyl compounds was generated by a diastereoselective concerted hetero‐Diels‐Alder cycloaddition.Instituto PolitĂ©cnico Nacional, Secretaria de InvestigaciĂłn y Estudios Avanzados de la Universidad AutĂłnoma del Estado de MĂ©xico, Universidad de Guanajuato y CONACYT

    POR UNA CULTURA DE PAZ: UNA MIRADA DESDE LAS CIENCIAS DE LA CONDUCTA

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    En
 virtud
 de
 lo
 anterior,
 los
 estudiosos
 de
 las
 ciencias
 de
 la
 conducta
 
de
 la
 Universidad
AutĂłnoma 
del
 Estado 
de 
MĂ©xico,

ante 
la
persistencia
 y 
proliferaciĂłn
 de
 estos 
hechos
 en
 diversas
 partes
 del
Mundo
 y
 de
 nuestro 
paĂ­s 
en 
particular, se
 convocó
 a
 los
 estudiosos
 interesados
 y
 a
 la
 sociedad
 en
 general
 a
 presentar
 trabajos
 para
 analizar,
 debatir
 y
 proponer
 estrategias
 de
 acciĂłn
 y
 direcciĂłn,
 que
 fortalezcan
 una
 convivencia y bienestar con sentido humanista para una cultura de paz. El
 presente
 texto
 es
 producto
 de 
esta convocatoria 
que
 recoge 
los
trabajos 
de 

los
 interesados 
en 
la
 temĂĄtica,

 de
 diferentes 
paĂ­ses
(España,
Argentina,
Cuba,
Brasil,
Costa
 Rica
 y
 MĂ©xico)
 retomando
 con
 ello
 sus
 experiencias
 relativas
 al
 estudio,
 anĂĄlisis,
 comprensiĂłn
 e
 instrumentaciĂłn
 de
 la
 cultura
 de
 paz
 en
 los
 distintos
 ĂĄmbitos
 institucionales
 en
 los
 que
 participan:
 educativo,
 salud,
 penitenciario,
 social,
laboral,
familia,
alimentario,
psicolĂłgico,
por 
mencionar 
algunos.
 El
 presente
 libro,
 propicia
 un
 espacio
 de
 reflexiĂłn,
 diĂĄlogo
 y
 posicionamiento
 de
 las 
ciencias 
de 
la 
conducta
 para 
la 
apropiaciĂłn,
anĂĄlisis,
debate
 y 
propuestas 
que
 fortalezcan 
una
 cultura
 de 
paz
 a
travĂ©s
 de 
la
 convivencia 
y
 el 
bienestar
 social 
con
 sentido 
humanista.
El
 sistema 
econĂłmico
 neoliberal
 y 
el 
proceso
 de 
globalizaciĂłn 
han
 contribuido
al
 logro
 de
 avances
 significativos
 en
 la
 ciencia
 y
 la
 tecnologĂ­a,
 pero
 tambiĂ©n
 han
 propiciado
 la
 polarizaciĂłn
 de
 las
 sociedades
 lo
 que
 ha
 impactado
 de
 manera
 negativa
 a
 la
 sociedad
 en
 su
 conjunto,
 pero
 en
 mayor
 medida
 a
 los grupos
 vulnerables. Dicha
 polarizaciĂłn
 ha
 traĂ­do
 consigo
 un
 desarrollo
 desigual
 del
 mundo
 que
 se
 expresa
 de
 diferentes
 maneras
 tanto
 en
 paĂ­ses
 desarrollados
 como
 en
 los
 llamados
 del
 tercer
 mundo,
 en
 donde
 no
 estĂĄn
 satisfechas
 las
 necesidades
 humanas 
elementales
 de
 todos 
los
sectores 
de 
la 
poblaciĂłn,
siempre 
falta 
algo. 
Si 
a
 esto 
le
 sumamos 
los
conflictos
 internacionales por
 diferentes
 motivos
 que
 enfrentan
 algunas
 naciones,
 una
 insuficiente
 cobertura
 educativa
 y
 de
 salud,

 desempleo
 y
 pobreza 
extrema,
 entre 
otras
 cosasÍŸ 
estamos
 frente
 a
retos 
de
 gran
 envergadura
 para
 los
 gobiernos,
 para
 los
 estudiosos
 y
 para
 la
 sociedad
 civil
 en
 general. Uno 
de 
los
 intentos
 para
 frenar 
y prevenir 
la
 agudizaciĂłn
 de 
estas 
problemĂĄticas
 es
 la
 cultura 
de 
paz,
cuyo
 estudio
y propuestas 
han 
ido 
avanzando 
en 
diferentes
 sentidos 
y 
de 
manera 
favorable,
el 
tema 
estĂĄ 
presente 
en 
diferentes 
Organismos
 Internacionales
 como
 la
 ONU,
 la
 UNESCO,
 la
 OCDE,
 El
 Banco
 Mundial,
 entre
 otros.
 Pero
 falta 
mucho 
por 
hacer.Universidad AutĂłnoma del Estado de MĂ©xic

    Taking the pulse of Earth's tropical forests using networks of highly distributed plots

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    Tropical forests are the most diverse and productive ecosystems on Earth. While better understanding of these forests is critical for our collective future, until quite recently efforts to measure and monitor them have been largely disconnected. Networking is essential to discover the answers to questions that transcend borders and the horizons of funding agencies. Here we show how a global community is responding to the challenges of tropical ecosystem research with diverse teams measuring forests tree-by-tree in thousands of long-term plots. We review the major scientific discoveries of this work and show how this process is changing tropical forest science. Our core approach involves linking long-term grassroots initiatives with standardized protocols and data management to generate robust scaled-up results. By connecting tropical researchers and elevating their status, our Social Research Network model recognises the key role of the data originator in scientific discovery. Conceived in 1999 with RAINFOR (South America), our permanent plot networks have been adapted to Africa (AfriTRON) and Southeast Asia (T-FORCES) and widely emulated worldwide. Now these multiple initiatives are integrated via ForestPlots.net cyber-infrastructure, linking colleagues from 54 countries across 24 plot networks. Collectively these are transforming understanding of tropical forests and their biospheric role. Together we have discovered how, where and why forest carbon and biodiversity are responding to climate change, and how they feedback on it. This long-term pan-tropical collaboration has revealed a large long-term carbon sink and its trends, as well as making clear which drivers are most important, which forest processes are affected, where they are changing, what the lags are, and the likely future responses of tropical forests as the climate continues to change. By leveraging a remarkably old technology, plot networks are sparking a very modern revolution in tropical forest science. In the future, humanity can benefit greatly by nurturing the grassroots communities now collectively capable of generating unique, long-term understanding of Earth's most precious forests. Resumen Los bosques tropicales son los ecosistemas mĂĄs diversos y productivos del mundo y entender su funcionamiento es crĂ­tico para nuestro futuro colectivo. Sin embargo, hasta hace muy poco, los esfuerzos para medirlos y monitorearlos han estado muy desconectados. El trabajo en redes es esencial para descubrir las respuestas a preguntas que trascienden las fronteras y los plazos de las agencias de financiamiento. AquĂ­ mostramos cĂłmo una comunidad global estĂĄ respondiendo a los desafĂ­os de la investigaciĂłn en ecosistemas tropicales a travĂ©s de diversos equipos realizando mediciones ĂĄrbol por ĂĄrbol en miles de parcelas permanentes de largo plazo. Revisamos los descubrimientos mĂĄs importantes de este trabajo y discutimos cĂłmo este proceso estĂĄ cambiando la ciencia relacionada a los bosques tropicales. El enfoque central de nuestro esfuerzo implica la conexiĂłn de iniciativas locales de largo plazo con protocolos estandarizados y manejo de datos para producir resultados que se puedan trasladar a mĂșltiples escalas. Conectando investigadores tropicales, elevando su posiciĂłn y estatus, nuestro modelo de Red Social de InvestigaciĂłn reconoce el rol fundamental que tienen, para el descubrimiento cientĂ­fico, quienes generan o producen los datos. Concebida en 1999 con RAINFOR (SuramĂ©rica), nuestras redes de parcelas permanentes han sido adaptadas en África (AfriTRON) y el sureste asiĂĄtico (T-FORCES) y ampliamente replicadas en el mundo. Actualmente todas estas iniciativas estĂĄn integradas a travĂ©s de la ciber-infraestructura de ForestPlots.net, conectando colegas de 54 paĂ­ses en 24 redes diferentes de parcelas. Colectivamente, estas redes estĂĄn transformando nuestro conocimiento sobre los bosques tropicales y el rol de Ă©stos en la biĂłsfera. Juntos hemos descubierto cĂłmo, dĂłnde y porquĂ© el carbono y la biodiversidad de los bosques tropicales estĂĄ respondiendo al cambio climĂĄtico y cĂłmo se retroalimentan. Esta colaboraciĂłn pan-tropical de largo plazo ha expuesto un gran sumidero de carbono y sus tendencias, mostrando claramente cuĂĄles son los factores mĂĄs importantes, quĂ© procesos se ven afectados, dĂłnde ocurren los cambios, los tiempos de reacciĂłn y las probables respuestas futuras mientras el clima continĂșa cambiando. Apalancando lo que realmente es una tecnologĂ­a antigua, las redes de parcelas estĂĄn generando una verdadera y moderna revoluciĂłn en la ciencia tropical. En el futuro, la humanidad puede beneficiarse enormemente si se nutren y cultivan comunidades de investigadores de base, actualmente con la capacidad de generar informaciĂłn Ășnica y de largo plazo para entender los que probablemente son los bosques mĂĄs preciados de la tierra. Resumo Florestas tropicais sĂŁo os ecossistemas mais diversos e produtivos da Terra. Embora uma boa compreensĂŁo destas florestas seja crucial para o nosso futuro coletivo, atĂ© muito recentemente os esforços de mediçÔes e monitoramento foram amplamente desconexos. É essencial formarmos redes para obtermos respostas que transcendem fronteiras e horizontes de agĂȘncias financiadoras. Neste estudo nĂłs mostramos como uma comunidade global estĂĄ respondendo aos desafios da pesquisa de ecossistemas tropicais, com equipes diversas medindo florestas, ĂĄrvore por ĂĄrvore, em milhares de parcelas monitoradas Ă  longo prazo. NĂłs revisamos as maiores descobertas cientĂ­ficas deste trabalho, e mostramos tambĂ©m como este processo estĂĄ mudando a ciĂȘncia de florestas tropicais. Nossa abordagem principal envolve unir iniciativas de base a protocolos padronizados e gerenciamento de dados a fim de gerar resultados robustos em escalas ampliadas. Ao conectar pesquisadores tropicais e elevar seus status, nosso modelo de Rede de Pesquisa Social reconhece o papel-chave do produtor dos dados na descoberta cientĂ­fica. Concebida em 1999 com o RAINFOR (AmĂ©rica do Sul), nossa rede de parcelas permanentes foi adaptada para África (AfriTRON) e Sudeste asiĂĄtico (T-FORCES), e tem sido extensamente reproduzida em todo o mundo. Agora estas mĂșltiplas iniciativas estĂŁo integradas atravĂ©s de uma infraestrutura cibernĂ©tica do ForestPlots.net, conectando colegas de 54 paĂ­ses de 24 redes de parcelas. Estas iniciativas estĂŁo transformando coletivamente o entendimento das florestas tropicais e seus papĂ©is na biosfera. Juntos nĂłs descobrimos como, onde e por que o carbono e a biodiversidade da floresta estĂŁo respondendo Ă s mudanças climĂĄticas, e seus efeitos de retroalimentação. Esta duradoura colaboração pantropical revelou um grande sumidouro de carbono persistente e suas tendĂȘncias, assim como tem evidenciado quais direcionadores sĂŁo mais importantes, quais processos florestais sĂŁo mais afetados, onde eles estĂŁo mudando, seus atrasos no tempo de resposta, e as provĂĄveis respostas das florestas tropicais conforme o clima continua a mudar. Dessa forma, aproveitando uma notĂĄvel tecnologia antiga, redes de parcelas acendem faĂ­scas de uma moderna revolução na ciĂȘncia das florestas tropicais. No futuro a humanidade pode se beneficiar incentivando estas comunidades basais que agora sĂŁo coletivamente capazes de gerar conhecimentos Ășnicos e duradouros sobre as florestas mais preciosas da Terra. RĂ©sume Les forĂȘts tropicales sont les Ă©cosystĂšmes les plus diversifiĂ©s et les plus productifs de la planĂšte. Si une meilleure comprĂ©hension de ces forĂȘts est essentielle pour notre avenir collectif, jusqu'Ă  tout rĂ©cemment, les efforts dĂ©ployĂ©s pour les mesurer et les surveiller ont Ă©tĂ© largement dĂ©connectĂ©s. La mise en rĂ©seau est essentielle pour dĂ©couvrir les rĂ©ponses Ă  des questions qui dĂ©passent les frontiĂšres et les horizons des organismes de financement. Nous montrons ici comment une communautĂ© mondiale relĂšve les dĂ©fis de la recherche sur les Ă©cosystĂšmes tropicaux avec diverses Ă©quipes qui mesurent les forĂȘts arbre aprĂšs arbre dans de milliers de parcelles permanentes. Nous passons en revue les principales dĂ©couvertes scientifiques de ces travaux et montrons comment ce processus modifie la science des forĂȘts tropicales. Notre approche principale consiste Ă  relier les initiatives de base Ă  long terme Ă  des protocoles standardisĂ©s et une gestion de donnĂ©es afin de gĂ©nĂ©rer des rĂ©sultats solides Ă  grande Ă©chelle. En reliant les chercheurs tropicaux et en Ă©levant leur statut, notre modĂšle de rĂ©seau de recherche sociale reconnaĂźt le rĂŽle clĂ© de l'auteur des donnĂ©es dans la dĂ©couverte scientifique. Conçus en 1999 avec RAINFOR (AmĂ©rique du Sud), nos rĂ©seaux de parcelles permanentes ont Ă©tĂ© adaptĂ©s Ă  l'Afrique (AfriTRON) et Ă  l'Asie du Sud-Est (T-FORCES) et largement imitĂ©s dans le monde entier. Ces multiples initiatives sont dĂ©sormais intĂ©grĂ©es via l'infrastructure ForestPlots.net, qui relie des collĂšgues de 54 pays Ă  travers 24 rĂ©seaux de parcelles. Ensemble, elles transforment la comprĂ©hension des forĂȘts tropicales et de leur rĂŽle biosphĂ©rique. Ensemble, nous avons dĂ©couvert comment, oĂč et pourquoi le carbone forestier et la biodiversitĂ© rĂ©agissent au changement climatique, et comment ils y rĂ©agissent. Cette collaboration pan-tropicale Ă  long terme a rĂ©vĂ©lĂ© un important puits de carbone Ă  long terme et ses tendances, tout en mettant en Ă©vidence les facteurs les plus importants, les processus forestiers qui sont affectĂ©s, les endroits oĂč ils changent, les dĂ©calages et les rĂ©actions futures probables des forĂȘts tropicales Ă  mesure que le climat continue de changer. En tirant parti d'une technologie remarquablement ancienne, les rĂ©seaux de parcelles dĂ©clenchent une rĂ©volution trĂšs moderne dans la science des forĂȘts tropicales. À l'avenir, l'humanitĂ© pourra grandement bĂ©nĂ©ficier du soutien des communautĂ©s de base qui sont maintenant collectivement capables de gĂ©nĂ©rer une comprĂ©hension unique et Ă  long terme des forĂȘts les plus prĂ©cieuses de la Terre. Abstrak Hutan tropika adalah di antara ekosistem yang paling produktif dan mempunyai kepelbagaian biodiversiti yang tinggi di seluruh dunia. Walaupun pemahaman mengenai hutan tropika amat penting untuk masa depan kita, usaha-usaha untuk mengkaji dan mengawas hutah-hutan tersebut baru sekarang menjadi lebih diperhubungkan. Perangkaian adalah sangat penting untuk mencari jawapan kepada soalan-soalan yang menjangkaui sempadan dan batasan agensi pendanaan. Di sini kami menunjukkan bagaimana sebuah komuniti global bertindak balas terhadap cabaran penyelidikan ekosistem tropika melalui penglibatan pelbagai kumpulan yang mengukur hutan secara pokok demi pokok dalam beribu-ribu plot jangka panjang. Kami meninjau semula penemuan saintifik utama daripada kerja ini dan menunjukkan bagaimana proses ini sedang mengubah bidang sains hutan tropika. Teras pendekatan kami memberi tumpuan terhadap penghubungan inisiatif akar umbi jangka panjang dengan protokol standar serta pengurusan data untuk mendapatkan hasil skala besar yang kukuh. Dengan menghubungkan penyelidik-penyelidik tropika dan meningkatkan status mereka, model Rangkaian Penyelidikan Sosial kami mengiktiraf kepentingan peranan pengasas data dalam penemuan saintifik. Bermula dengan pengasasan RAINFOR (Amerika Selatan) pada tahun 1999, rangkaian-rangkaian plot kekal kami kemudian disesuaikan untuk Afrika (AfriTRON) dan Asia Tenggara (T-FORCES) dan selanjutnya telah banyak dicontohi di seluruh dunia. Kini, inisiatif-inisiatif tersebut disepadukan melalui infrastruktur siber ForestPlots.net yang menghubungkan rakan sekerja dari 54 negara di 24 buah rangkaian plot. Secara kolektif, rangkaian ini sedang mengubah pemahaman tentang hutan tropika dan peranannya dalam biosfera. Kami telah bekerjasama untuk menemukan bagaimana, di mana dan mengapa karbon serta biodiversiti hutan bertindak balas terhadap perubahan iklim dan juga bagaimana mereka saling bermaklum balas. Kolaborasi pan-tropika jangka panjang ini telah mendedahkan sebuah sinki karbon jangka panjang serta arah alirannya dan juga menjelaskan pemandu-pemandu perubahan yang terpenting, di mana dan bagaimana proses hutan terjejas, masa susul yang ada dan kemungkinan tindakbalas hutan tropika pada perubahan iklim secara berterusan di masa depan. Dengan memanfaatkan pendekatan lama, rangkaian plot sedang menyalakan revolusi yang amat moden dalam sains hutan tropika. Pada masa akan datang, manusia sejagat akan banyak mendapat manfaat jika memupuk komuniti-komuniti akar umbi yang kini berkemampuan secara kolektif menghasilkan pemahaman unik dan jangka panjang mengenai hutan-hutan yang paling berharga di dunia

    Centrality evolution of the charged-particle pseudorapidity density over a broad pseudorapidity range in Pb-Pb collisions at root s(NN)=2.76TeV

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    Peer reviewe

    Mortality of emergency abdominal surgery in high-, middle- and low-income countries

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    Background: Surgical mortality data are collected routinely in high-income countries, yet virtually no low- or middle-income countries have outcome surveillance in place. The aim was prospectively to collect worldwide mortality data following emergency abdominal surgery, comparing findings across countries with a low, middle or high Human Development Index (HDI). Methods: This was a prospective, multicentre, cohort study. Self-selected hospitals performing emergency surgery submitted prespecified data for consecutive patients from at least one 2-week interval during July to December 2014. Postoperative mortality was analysed by hierarchical multivariable logistic regression. Results: Data were obtained for 10 745 patients from 357 centres in 58 countries; 6538 were from high-, 2889 from middle- and 1318 from low-HDI settings. The overall mortality rate was 1⋅6 per cent at 24 h (high 1⋅1 per cent, middle 1⋅9 per cent, low 3⋅4 per cent; P < 0⋅001), increasing to 5⋅4 per cent by 30 days (high 4⋅5 per cent, middle 6⋅0 per cent, low 8⋅6 per cent; P < 0⋅001). Of the 578 patients who died, 404 (69⋅9 per cent) did so between 24 h and 30 days following surgery (high 74⋅2 per cent, middle 68⋅8 per cent, low 60⋅5 per cent). After adjustment, 30-day mortality remained higher in middle-income (odds ratio (OR) 2⋅78, 95 per cent c.i. 1⋅84 to 4⋅20) and low-income (OR 2⋅97, 1⋅84 to 4⋅81) countries. Surgical safety checklist use was less frequent in low- and middle-income countries, but when used was associated with reduced mortality at 30 days. Conclusion: Mortality is three times higher in low- compared with high-HDI countries even when adjusted for prognostic factors. Patient safety factors may have an important role. Registration number: NCT02179112 (http://www.clinicaltrials.gov)

    High levels of glucose alter Physcomitrella patens metabolism and trigger a differential proteomic response.

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    Sugars act not only as substrates for plant metabolism, but also have a pivotal role in signaling pathways. Glucose signaling has been widely studied in the vascular plant Arabidopsis thaliana, but it has remained unexplored in non-vascular species such as Physcomitrella patens. To investigate P. patens response to high glucose treatment, we explored the dynamic changes in metabolism and protein population by applying a metabolomic fingerprint analysis (DIESI-MS), carbohydrate and chlorophyll quantification, Fv/Fm determination and label-free untargeted proteomics. Glucose feeding causes specific changes in P. patens metabolomic fingerprint, carbohydrate contents and protein accumulation, which is clearly different from those of osmotically induced responses. The maximal rate of PSII was not affected although chlorophyll decreased in both treatments. The biological process, cellular component, and molecular function gene ontology (GO) classifications of the differentially expressed proteins indicate the translation process is the most represented category in response to glucose, followed by photosynthesis, cellular response to oxidative stress and protein refolding. Importantly, although several proteins have high fold changes, these proteins have no predicted identity. The most significant discovery of our study at the proteome level is that high glucose increase abundance of proteins related to the translation process, which was not previously evidenced in non-vascular plants, indicating that regulation by glucose at the translational level is a partially conserved response in both plant lineages. To our knowledge, this is the first time that metabolome fingerprint and proteomic analyses are performed after a high sugar treatment in non-vascular plants. These findings unravel evolutionarily shared and differential responses between vascular and non-vascular plants

    Activity of Chloroformic Extract from <i>Salvia connivens</i> (Lamiales: Lamiaceae) and Its Principal Compounds against <i>Spodoptera frugiperda</i> (Lepidoptera: Noctuidae)

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    Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae) is one of the most damaging pests in maize crops. In order to manage it, synthetic insecticides such as diamides, neonicotinoids, and pyrethroids are used, but they present a risk for humans and the environment. Investigations of safer alternatives include the use of natural extracts. Thus, this research evaluated the effects of chloroform extract (CHCl3Sc) (5000, 4000, 2000, 1000, and 500 ppm) on aerial parts of Salvia connivens and of nonanal and pyrocatechol (1000, 600, 400, and 80 ppm) on S. frugiperda mortality, duration of the larva and pupae phases, and pupae weight after 24 h. The second instars of S. frugiperda larvae were fed an artificial diet incorporating the extract and compounds. The CHCl3Sc had insecticidal activity against S. frugiperda, showing an LC50 of 1504 ppm. Insectistatic activity began at 1000 ppm, increasing pupal and larval duration in 7.6 and 1.4 days, respectively. Pyrocatechol and nonanal were found in this extract. The first did not have any significant difference in larval or pupal mortalities. On the other hand, insectistatic activity was shown at 500 ppm, increasing the larval duration by 1.7 days compared with the control. In the case of nonanal, the insecticide activity was LC50 of 200 ppm, and insectistatic activity started at 80 ppm, increasing larval duration by 3.2 days compared with the control and reducing pupal weight by 3.4%. The results show that chloroformic extract had insecticidal and insectistatic activities against S. frugiperda; nonanal was an aldehyde compound present in this extract, which confers insecticidal and insectistatic activities against this pest

    De novo assembly of maritime pine transcriptome: implications for forest breeding and biotechnology

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    Maritime pine (Pinus pinasterAit.) is a widely distributed conifer species in Southwestern Europe and one of the most advanced models for conifer research. In the current work, comprehensive characterization of the maritime pine transcriptome was performed using a combination of two different next-generation sequencing platforms, 454 and Illumina. De novo assembly of the transcriptome provided a catalogue of 26 020 unique transcripts in maritime pine trees and a collection of 9641 full-length cDNAs. Quality of the transcriptome assembly was validated by RT-PCR amplification of selected transcripts for structural and regulatory genes. Transcription factors and enzyme-encoding transcripts were annotated. Furthermore, the available sequencing data permitted the identification of polymorphisms and the establishment of robust single nucleotide polymorphism (SNP) and simple-sequence repeat (SSR) databases for genotyping applications and integration of translational genomics in maritime pine breeding programmes. All our data are freely available at SustainpineDB, the P. pinaster expressional database. Results reported here on the maritime pine transcriptome represent a valuable resource for future basic and applied studies on this ecological and economically important pine species
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